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KMID : 0350519940470041749
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Journal of Catholic Medical College
1994 Volume.47 No. 4 p.1749 ~ p.1759
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HLA Class I and ⅡGenes in Korean Children with Graves' Disease
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Abstract
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Graves' disease is a typical organ-specific autoimmune disease, and antigens of the human major histocompatibility loci have been reported to function as marker for hereditary susceptibility in several disorders of autoimmunity.
To investigate HLA types in Korean children with Graves' disease, we studied the HLA class I and ii genes by the standard microlymphocytotoxicity technique for class I and the two step polymerase chain reaction for class ii in 40 Korean children
with
Graves' disease and 200 normal healthy children.
After HLA-A, B, C typings by the standard microlymphocytotoxicity technique, we designed 12 pairs of group specific primers followed by the alleles which have the same oligonucleotide sequences in the oxon 2 hypervariable region. We performed
amplification reaction with these pairs of primers for 32 cycles. Reaction products were electrophoresed and then only specifically amplified products were reamplified for 15 cycles with allele specific primers for the analysis of HLA-DR and DQ
genes
with specific band patterns of each alleles.
The HLA class I and ii genotypes in patients were compared to the data from normal children.
@ES The results were as follows:
@EN 1. The frequency of HLA-A2(77.5%) in the patients with Graves' disease was significantly higher than that in normal control group(55.5%) and the relative risk was 2.76.
2. The frequency of HLA-B46(22.5%) in the patients with Graves' Graves' disease was significantly higher than that in normal control group(5.5%) and the relative risk was 4.99.
3. The frequencies of HLA-Cw1(37.5%) and Cw3(70.0%) in the patients with Graves' disease tend to be higher than those in normal control group(24.5 and 63.5%).
4. The frequency of HLA-DR8(52.5%) in the patients with Graves' disease was significantly higher than that in normal control group(16.9%) and the relative risk was 5.43.
5. The frequencies of HLA-DQw6(47.5%) and DQw4(22.5%) in the patients with Graves' disease were significantly higher than those in normal control group(4.4 and 22.5%) and both relative risks were 19.60 and 7.61.
These results suggested that the frequency of HLA-DQw6, DQw4, DR8, B46 and A2 was significantly increased in Korean children with Graves' disease.
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KEYWORD
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